Nowadays, short-read RNA sequencing (RNA-seq) is the most widely used high-throughput method and has greatly expanded our ability to study transcriptome complexity, such as predicting alternative splicing events. However, short-read RNA-seq has many inherent limitations and cannot accurately determine all isoforms. pacbio rna seq https://longseq.cd-genomics.co....m/pacbio-rna-sequenc
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